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p2x1 receptor blocking peptide  (Alomone Labs)


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    Structured Review

    Alomone Labs p2x1 receptor blocking peptide
    P2x1 Receptor Blocking Peptide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p2x1+receptor+blocking+peptide/P2X1+Receptor+Blocking+Peptide/pm41579306-40-6-10
    Average 91 stars, based on 8 article reviews
    p2x1 receptor blocking peptide - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Control:

    Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea.
    Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution). .. For the peptide preabsorption control, the P2X1 receptor blocking peptide (Alomone Labs, Jerusalem, Israel, Catalogue # BLP-PR001) was mixed with the primary antibody in a 1 mg to 1 mg ratio and incubated for one hour at room temperature following the manufacturer’s protocol. .. Secondary antibodies used were: goat polyclonal anti-rabbit Alexa 594, goat polyclonal anti-mouse Alexa 488, donkey polyclonal anti-rabbit DyLight 594, donkey polyclonal anti-goat Dylight 488 (ThermoFisher, all used at 1:1000 dilutions).

    Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea
    Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution). .. For the peptide preabsorption control, the P2X1 receptor blocking peptide (Alomone Labs, Jerusalem, Israel, Catalogue # BLP-PR001) was mixed with the primary antibody in a 1 mg to 1 mg ratio and incubated for one hour at room temperature following the manufacturer’s protocol. .. Secondary antibodies used were: goat polyclonal anti-rabbit Alexa 594, goat polyclonal anti-mouse Alexa 488, donkey polyclonal anti-rabbit DyLight 594, donkey polyclonal anti-goat Dylight 488 (ThermoFisher, all used at 1:1000 dilutions).

    Blocking Assay:

    Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea.
    Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution). .. For the peptide preabsorption control, the P2X1 receptor blocking peptide (Alomone Labs, Jerusalem, Israel, Catalogue # BLP-PR001) was mixed with the primary antibody in a 1 mg to 1 mg ratio and incubated for one hour at room temperature following the manufacturer’s protocol. .. Secondary antibodies used were: goat polyclonal anti-rabbit Alexa 594, goat polyclonal anti-mouse Alexa 488, donkey polyclonal anti-rabbit DyLight 594, donkey polyclonal anti-goat Dylight 488 (ThermoFisher, all used at 1:1000 dilutions).

    Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea
    Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution). .. For the peptide preabsorption control, the P2X1 receptor blocking peptide (Alomone Labs, Jerusalem, Israel, Catalogue # BLP-PR001) was mixed with the primary antibody in a 1 mg to 1 mg ratio and incubated for one hour at room temperature following the manufacturer’s protocol. .. Secondary antibodies used were: goat polyclonal anti-rabbit Alexa 594, goat polyclonal anti-mouse Alexa 488, donkey polyclonal anti-rabbit DyLight 594, donkey polyclonal anti-goat Dylight 488 (ThermoFisher, all used at 1:1000 dilutions).

    Incubation:

    Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea.
    Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution). .. For the peptide preabsorption control, the P2X1 receptor blocking peptide (Alomone Labs, Jerusalem, Israel, Catalogue # BLP-PR001) was mixed with the primary antibody in a 1 mg to 1 mg ratio and incubated for one hour at room temperature following the manufacturer’s protocol. .. Secondary antibodies used were: goat polyclonal anti-rabbit Alexa 594, goat polyclonal anti-mouse Alexa 488, donkey polyclonal anti-rabbit DyLight 594, donkey polyclonal anti-goat Dylight 488 (ThermoFisher, all used at 1:1000 dilutions).

    Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea
    Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution). .. For the peptide preabsorption control, the P2X1 receptor blocking peptide (Alomone Labs, Jerusalem, Israel, Catalogue # BLP-PR001) was mixed with the primary antibody in a 1 mg to 1 mg ratio and incubated for one hour at room temperature following the manufacturer’s protocol. .. Secondary antibodies used were: goat polyclonal anti-rabbit Alexa 594, goat polyclonal anti-mouse Alexa 488, donkey polyclonal anti-rabbit DyLight 594, donkey polyclonal anti-goat Dylight 488 (ThermoFisher, all used at 1:1000 dilutions).



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    Image Search Results


    Western blot analysis with specific antibodies to the TRPV1 receptor, purinergic P2X 2 receptor, and P2X 3 receptor of the rat mucosal layer and the purinergic P2X 1 receptor of the rat smooth muscle layer in controls and FFRs with different in cystometric presentations. A. TRPV1 receptor: The TRPV1 antibody produced a clear single band at 95kDa. B. Purinergic P2X 2 receptor C. Purinergic P2X 3 mature receptor: the predominant P2X 3 form (65kDa). D. Up-regulation of P2X3 native and intermediate polypeptides (up to 55kD) were shown in both FFR groups. E. Purinergic P2X 1 receptor Experiments were repeated two times and representative blots are shown. Data of proteins expression (ratios of signal intensities of investigated receptors relative to β-actin or GAPDH) were calculated with 8 samples in each group. These data of Mean ± SE were standardized and expressed in percentage in which the value of the control group is treated as 100%. Theses values were shown in the bar graph. An asterisk indicates a significant difference between controls and FFR groups (One-way ANOVA with Dunnett’s test, p<0.05).

    Journal: PLoS ONE

    Article Title: Sensory Dysfunction of Bladder Mucosa and Bladder Oversensitivity in a Rat Model of Metabolic Syndrome

    doi: 10.1371/journal.pone.0045578

    Figure Lengend Snippet: Western blot analysis with specific antibodies to the TRPV1 receptor, purinergic P2X 2 receptor, and P2X 3 receptor of the rat mucosal layer and the purinergic P2X 1 receptor of the rat smooth muscle layer in controls and FFRs with different in cystometric presentations. A. TRPV1 receptor: The TRPV1 antibody produced a clear single band at 95kDa. B. Purinergic P2X 2 receptor C. Purinergic P2X 3 mature receptor: the predominant P2X 3 form (65kDa). D. Up-regulation of P2X3 native and intermediate polypeptides (up to 55kD) were shown in both FFR groups. E. Purinergic P2X 1 receptor Experiments were repeated two times and representative blots are shown. Data of proteins expression (ratios of signal intensities of investigated receptors relative to β-actin or GAPDH) were calculated with 8 samples in each group. These data of Mean ± SE were standardized and expressed in percentage in which the value of the control group is treated as 100%. Theses values were shown in the bar graph. An asterisk indicates a significant difference between controls and FFR groups (One-way ANOVA with Dunnett’s test, p<0.05).

    Article Snippet: Antibodies raised against TRPV1 receptor (Alomone, Israel; 1∶1000 dilution), P2X 2 receptor (Alomone, Israel; 1∶500 dilution), P2X 3 receptor (Neuromics, MN; 1∶500 dilution), P2X 1 receptor (Alomone, Israel; 1∶500 dilution), iNOS (Cayman Chemical, Michigan; 1∶500 dilution), eNOS (BD Biosciences, CA; 1∶1000 dilution), GAPDH (Millipore, CA; 1∶10000 dilution ) and β-actin (Millipore, CA; 1∶10000 dilution) were used.

    Techniques: Western Blot, Produced, Expressing